Journal: Communications Biology
Article Title: Multiplexed proteomic biosensor platform for label-free real-time simultaneous kinetic screening of thousands of protein interactions
doi: 10.1038/s42003-025-07844-z
Figure Lengend Snippet: a Image of the SPOC sensor surface (10 k pattern) as viewed from the flowcell of the OpenPleX. The hockey-stick shaped orientation mark is visible in the center of the array. b Difference image showing the pattern of binding observed on the SPOC biosensor surface after injection of mouse anti-HaloTag antibody (133 nM). c Difference image showing the pattern of binding observed after the Mouse anti-Jun antibody injection (200 nM) which specifically detects two spots (yellow marks) where Jun-HaloTag was capture-purified as expected. d 1:1 fit (orange line) OpenPleX data of the anti-Jun antibody binding to Jun Spots #1 and #2 from c show similar binding profiles and equilibrium dissociation constants. e Difference image shows the pattern of binding observed after the mouse anti-p53 antibody injection (17.8 nM) which specifically detects four spots (yellow marks) where p53-HaloTag was capture-purified as expected. f 1:1 fit (orange lines) OpenPleX data of the anti-p53 antibody binding to the four p53 spots detected in e show similar binding profiles and equilibrium dissociation constants. g Iso-affinity plot of the kinetics measured for the Mouse anti-p53 and anti-Jun injections (p53 = 10 spots across 3 sensors; Jun = 4 spots across 2 sensors). The numerical source dataset for this iso-affinity plot is on Table .
Article Snippet: The SPOC technology is adaptable to any SPR instrument; however, for this publication, the custom Carterra LSA XT SPR and Horiba Scientific OpenPleX SPR imaging (SPRi) instruments were used for validation because both instruments are amenable to HTP screening and require minimal effort to integrate with our SPOC protein biosensor platform.
Techniques: Binding Assay, Injection, Purification
